DNA is commonly extracted from biological tissues using a method that involves phenol/chloroform treatment followed by precipitation. However, this method requires the use of phenol and chloroform, both of which are classified as hazardous substances.
DNA Extractor® Kit series uses sodium iodide, a chaotropic agent, instead of organic solvents for DNA extraction.
Sodium iodide exhibits both protein-denaturing and solubilizing effects. It releases chaotropic ions (I-) into solution, which increase the solubility of hydrophobic molecules and weaken hydrophobic interactions. These effects contribute particularly to the denaturation and solubilization of membrane proteins, such as those found in the nuclear membrane.
Furthermore, because this protocol does not involve solid-phase extraction using silica carriers, DNA loss due to adsorption is minimized. In addition, performing all steps in a single tube helps ensure high recovery rates.