Extracellular vesicles (EVs), including exosomes, are prone to decrease in quantity during experimental procedures for several reasons. Especially, adsorption to tubes and other surfaces is a significant factor in the loss of EVs. The MISEV2023 guidelines also mention that purified EVs are prone to adsorption to storage containers1).
Using low protein-binding tubes can be effective in preventing the adsorption of EVs to tubes2-3). However, the plastic products used in experiments are not limited to tubes, but also include pipette tips, centrifugal concentrator tubes, etc., and it is impractical to pretreat all these consumables for low adsorption.

In such situations, a blocking agent added to samples or purified EV solutions offers an excellent solution in terms of operability and versatility. The EV-Save™ Extracellular Vesicle Blocking Reagent (Product Number: 058-09261), which contains a polymer, can be added to samples or purified EV solutions to prevent their adsorption to tubes, pipette tips, and centrifugal concentrator tubes. Furthermore, EV-Save™ acts as a cryoprotectant, minimizing damage to EVs during freeze-thaw cycles. Fujifilm Wako has also developed EV-Save™ which is specialized for in vivo experiment and EV purification by Tangential flow filtration (TFF).