rBC2LCN (AiLecS1) is a recombinant lectin produced by expressing the N-terminal domain of BC2L-C, a lectin derived from Burkholderia cenocepacia, in Escherichia coli. rBC2LCN binds with high affinity to H type 3 (Fucα1-2Galβ1-3GalNAc), a mucin-type O-glycan present on a protein called podocalyxin. Because podocalyxin is found on the surface of human ES/iPS cells, it has been reported that rBC2LCN can be employed as a marker for undifferentiated human ES/iPS cells.
Labeled with a fluorochrome, this product can simply be added to the culture solution to analyze live undifferentiated ES/iPS cells. It is compatible with both cell staining and flow cytometry. No cytotoxicity has been detected in continuous culture for several days in the presence of this product.
The product lineup offers two types of fluorescent labels: green (fluorescein isothiocyanate) and red (Cy5 spectrum).