This product is a fluorescently labeled recombinant lectin, rBC2LCN (AiLecS1), which shows strong affinity for undifferentiated cells.
rBC2LCN is produced in E. coli and corresponds to the N-terminal domain of BC2L-C, a lectin derived from Burkholderia cenocepacia. BC2LCN binds with exceptionally high affinity to H-type 3 (Fucα1-2Galβ1-3GalNAc), a mucin-type O-glycan present on the surface of undifferentiated human ES/iPS cells. Accordingly, it serves as a useful marker for identifying undifferentiated human ES/iPS cells.
Because this product is fluorescently labeled, undifferentiated human ES/iPS cells can be detected in live cultures simply by adding it to the medium at an appropriate concentration. It is compatible with both cell staining and flow cytometry. Furthermore, no cytotoxicity has been observed, even after several days of culture in the presence of this product.
This product is free of animal-derived components. Therefore, it is suitable for confirming undifferentiated status or removing undifferentiated human ES/iPS cells by flow cytometry during the manufacture of regenerative medicine products derived from human ES/iPS cells.