DISPASE is a metalloprotease that cleaves the N-terminal of neutral nonpolar amino acids in peptide chains. It detaches epithelial cells from tissues in a sheet-like structure and has long been used for cell separation and dispersion for primary culture. In addition, DISPASE, which is characterized by lower cytotoxicity and gentler cell dispersion than trypsin, collagenase, and other proteases, is also used in regenerative medicine such as ES/iPS cells.