In proteomics, the protein extraction and digestion, the preparatory steps for LC-MS/MS, play a crucial role in determining experimental results. Membrane proteins, in particular, are notoriously difficult to extract due to their high hydrophobicity. Membrane protein extraction in proteomics often necessitates the use of powerful solubilizing agents. However, such agents can inhibit the activity of proteolytic enzymes in subsequent steps and negatively affect peptide separation and ion detection in LC-MS/MS.
LysoPure™ Protein Extraction-PTS Solution is a solubilizing agent designed for protein extraction, an essential pretreatment step in proteomics.
Developed based on the PTS method, this product effectively solubilizes difficult-to-extract proteins while preserving proteolytic enzyme activity and enables easy removal of the agent via liquid-liquid partitioning. This product is an improved version of previously reported PTS solutions, offering improved protein solubilization and greater proteolytic enzyme activity than conventional PTS solutions. Additionally, it reduces cleavage errors by proteolytic enzymes. This product can also be used in proteomic studies of extracellular vesicles, including exosomes.