This product was developed as a nucleic acid reagent to replace ethidium bromide, which is known to be highly mutagenic. SAFELOOK™ Green/Red Nucleic Acid Stain can be used both pre- and post-staining. SAFELOOK™ Load-Green/Load-Red is mixed with samples at the time of use.
| SAFELOOK™ Green Nucleic Acid Stain | SAFELOOK™ Red Nucleic Acid Stain | SAFELOOK™ Load-Green (6×) | SAFELOOK™ Load-Red (6×) | |
| Staining method | Pre-/Post-staining | Pre-/Post-staining | Addition to samples | Addition to samples |
| Excitation wavelength | 490 nm | 540 nm | 490 nm | 540 nm |
| Fluorescence wavelength | 520 nm（DNA） 635 nm（RNA） | 630 nm | 525 nm | 630 nm |
| Light source | LED*1 / UV | LED / UV*2 | LED*1 / UV | LED / UV*2 |
| Recommended gel | Agarose | Agarose | Agarose/Polyacrylamide | Agarose/Polyacrylamide |
| Recommended sample | dsDNA / ssDNA / RNA | dsDNA / ssDNA / RNA | dsDNA / ssDNA / RNA | dsDNA / ssDNA / RNA |
Add 5 µL of SAFELOOK™ per 100 mL of gel solution. As required, add a further 5-10 µL to 200 mL of electrophoresis buffer.
Add 10-20 µL of SAFELOOK™ per 100 mL of buffer (1:5000-1:10000).
Add SAFELOOK™ to samples/markers in the ratio of 1:5.