Thin layer chromatography (TLC) is a method that uses a thin layer made of a stationary phase such as silica gel, polyamide, alumina, etc. on a glass or aluminum plate, separates the mixture sample into each component with a mobile phase. TLC is widely used for substance identification, purity testing, and for examining separation conditions when using column chromatography. Fujifilm Wako provides Silica Gel TLC Plates (Unmodified), NH2 modified Silica Gel Plates, and TLC plates, which thin layer is made from polyamide.
"NH2 Silica Gel 60F254 Plate-Wako" is a thin-layer chromatography plate coated with aminopropyl-modified silica gel on a glass plate. By irradiating the UV (λ=254 nm), the separated spots can be observed as black on a green background. Fujifilm Wako provides the NH2 Silica Gel 60F254 Plate-Wako with three kind of layer thickness, 0.25 mm type, which is suitable for examining separation conditions, 0.5 mm and 0.75 mm type, which are suitable for preparative separation.