Thin layer chromatography (TLC) is a method that uses a thin layer made of a stationary phase such as silica gel, polyamide, alumina, etc. on a glass or aluminum plate, separates the mixture sample into each component with a mobile phase. TLC is widely used for substance identification, purity testing, and for examining separation conditions when using column chromatography. Fujifilm Wako provides Silica Gel TLC Plates (Unmodified), NH2 modified Silica Gel TLC Plates, and TLC plates, which thin layer is made from polyamide.
Polyamide FM Plates are TLC plates that use polyamide as a carrier and add three types of fluorescent substances mixture (red, green, and blue). Polyamides are used in adsorption chromatography mode and are suitable for the separation of phenols and carboxylic acids, making them ideal for the analysis of preservatives, fungicides, antioxidants, sweeteners, and colorants in foods. In addition, due to the addition of three types of fluorescent substances mixture, the Polyamide FM Plate shows a substance-specific ultraviolet absorption when irradiated with a wide range of ultraviolet rays (250 to 400 nm), so the target substance can be observed as a colored spot such as red or blue.